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. 2013 Sep 2;8(9):e72625. doi: 10.1371/journal.pone.0072625

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© 2013 Maranhão et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Individual phage particles derived from chosen V4 and V10 pools were tested. Phage clones with HCDR3 peptides in the context of V4 and V10 were chosen. Phage supernatant was transferred to oligo-dT adsorbed microplates and serially diluted. Phages were detected with anti-M13 antibodies. Blue, V4 phage clones; red, V10 clones. Symbols differentiate HCDR3: diamonds, YLLSPLLLA, circle, VQQVNNLA e triangle VQYVNNALA. Green line represents the VCM13 helper phage as the negative binding control.

Inline graphic — Individual phage particles derived from chosen V4 and V10 pools were tested. Phage clones with HCDR3 peptides in the context of V4 and V10 were chosen. Phage supernatant was transferred to oligo-dT adsorbed microplates and serially diluted. Phages were detected with anti-M13 antibodies. Blue, V4 phage clones; red, V10 clones. Symbols differentiate HCDR3: diamonds, YLLSPLLLA, circle, VQQVNNLA e triangle VQYVNNALA. Green line represents the VCM13 helper phage as the negative binding control.