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. 2013 Sep 2;8(9):e71969. doi: 10.1371/journal.pone.0071969

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© 2013 Yoshikawa et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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a, Western blotting for the indicated genes in isolated colon epithelial cells in bulk after induction of colitis-associated tumors. Representative images of two independent mouse sets are shown. The band intensities were quantitated and adjusted by the expression levels of β-actin. The calculated ratios are indicated below each panel after setting the value of the control mouse as 1.0. b, Immunohistochemical analyses of the indicated protein expression in the tumor and surrounding non-tumor tissue from control and Dicer1-mutant mice after induction of colitis-associated tumors. Scale bars = 200 μm. Representative images are shown. Similar results were obtained from four independent mice per group.