Table 3. Partial ¹H chemical shift assignments (in ppm) for residues 1–7 of SP-B, as assigned based on 2D TOCSY and NOESY NMR experiments with SP-B (1–7) and SP-B (1–25,63–78) (Super Mini-B) in deuterated SDS at 45°C.

Residue	NH	Hα	Hβ	Hother
F1	7.66	4.52	3.45, 3.24	Hδ = 7.67, Hε = 7.32 Hζ = 7.55
P2/4/6	N/A	3.85	1.78, 2.12	Hγ = 1.29,1.55, Hδ = 3.66,3.18,
P2/4/6	N/A	4.38	1.77, 2.28	Hγ = 1.94,1.78, Hδ = 3.47,3.24
P2/4/6	N/A	4.53	2.04, 2.14	Hγ = 1.54,1.16, Hδ = 3.79,3.45
I3	unassigned	3.65	1.74	Hγ2 = 0.99
L5	7.31	4.52	2.05, 2.22	Hγ = 1.91, Hδ = 0.94,0.95
Y6	7.10	4.51	3.07, 2.74	Hδ = 7.10, Hε = 6.96

Note that although three sets of proline resonances were identified, none of them could be identified with a particular proline in the sequence.