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. 2013 Sep 2;8(9):e72858. doi: 10.1371/journal.pone.0072858

Figure 3. Simvastatin increases irisin secretion and FNDC5 mRNA expression in human skeletal muscle cells (HSKMCs).

Figure 3

Measurement of irisin in media (A) and lysate (B) at 24 and 48 hrs after 5 µM simvastatin treatment. FNDC5 mRNA levels were measured by real-time PCR after 5 µM simvastatin treatment for 6 and 24 hrs (C) or 1 and 5 µM for 24 hrs (D). mRNA levels of PGC-1α (E) and atrogin-1 (F) were also measured in simvastatin-treated HSKMCs (5 µM, 6 and 24 hrs). Values are means ± SEM of 4 individual experiments. *P<0.05 vs. control.