Table 2. Stage-specific effects of inhibitors.

Post synchronization stage at which treatment started	% Rings (R) at 48 hours and 60 hours post-synchronization in cultures containing DMSO (control) or inhibitors
	aControl	E64	MG132	Epoxomicin
	48/60 hours	48/60 hours	48/60 hours	48/60 hours
0 Hour	41 (±15)/80 (±12)	5 (±3)/5 (±0)	0/0	0/0
12 Hour	-	6 (±6)/5 (±2)	0/0	0/0
24 Hour	-	6 (±6)/6 (±1)	0/0	0/0
36 Hour	-	11 (±3)/12 (±0)	3 (±3)/10 (±0)	6 (±6)/0

Ring stage parasites were treated with DMSO (control) or inhibitors (21.7 µM E64, 0.024 µM epoxomicin, and 0.133 µM MG132; all at concentrations nearly 3 times the IC₅₀) at different stages of development (ring, T0 and T12; early trophozoite, T24; late trophozoite, T36), and the number of rings was counted at the end (48 hours) and 12 hours after the end (60 hours) of the development cycle. At least 200 parasite-infected cells were observed, and the table shows the percentage of rings for control and the inhibitor-treated cultures. The data are mean with standard deviation from three (for 48 hour) and two independent experiments (60 hour) done in duplicates.

^a DMSO was added at the start of the experiment (T0) only.