Table 1. Identified Tomato Proteins Involved in Pathogen-Tomato Interaction.
| Spot | Protein name | Accession Number | Organism | Protein score a | Mr (kDa) | PI | NUMP b | Fold c |
| Response to stress | ||||||||
| 1 | 17.7 kD class I small heat shock protein | gi|4836469 | Solanum lycopersicum | 174(65) | 17724 | 5.84 | 11 | 2.839 |
| 2 | heat shock protein 70 | gi|350537379 | Solanum lycopersicum | 380(53) | 74243.5 | 5.41 | 20 | 0.297 |
| 17 | small heat shock protein | gi|8918494 | Solanum lycopersicum | 349(53) | 21466.3 | 5.89 | 14 | 2.777 |
| 18 | pathogenesis-related protein PR P23 | gi|19315 | Solanum lycopersicum | 163(53) | 25111.5 | 6.13 | 8 | 3.102 |
| 23 | beta-1,3-glucanase | gi|170380 | Solanum lycopersicum | 71(53) | 37572 | 6.6 | 5 | 5.860 |
| 30 | acidic 26 kDa endochitinase precursor | gi|350534584 | Solanum lycopersicum | 468(53) | 27614.3 | 5.93 | 15 | 2.785 |
| 36 | class I small heat shock protein 17.6 | gi|349591295 | Solanum lycopersicum | 388(53) | 17634 | 5.84 | 19 | 0.067 |
| 41 | cytosolic class II small heat shock protein HCT2 | gi|3639075 | Solanum lycopersicum | 116(53) | 17321.9 | 6.75 | 8 | 3.216 |
| 40 | pathogenesis-related protein PR P23 | gi|19315 | Solanum lycopersicum | 134(53) | 25111.5 | 6.13 | 13 | 7.594 |
| 42 | STH-2 protein | gi|169576 | Solanum tuberosum | 346(59) | 17283.8 | 5.66 | 20 | 12.71 |
| 44 | cytosolic ascorbate peroxidase 1 | gi|73761751 | Solanum lycopersicum | 142(53) | 27390.8 | 5.61 | 21 | 3.853 |
| Protein metabolic process | ||||||||
| 11 | RAD23-like | gi|77745475 | Solanum tuberosum | 260(59) | 40607.5 | 4.71 | 12 | 0.361 |
| 22 | 26S protease regulatory subunit 6A homolog | gi|1729860 | Solanum lycopersicum | 261(59) | 47475.4 | 4.94 | 24 | 0.396 |
| 33 | Skp1-like protein 3 | gi|82470777 | Petunia integrifolia subsp. inflata | 67(59) | 17574.7 | 4.61 | 12 | 0.452 |
| 39 | proteasome-like protein alpha subunit | gi|77999303 | Solanum tuberosum | 86(59) | 27121.6 | 5.63 | 21 | 3.634 |
| 43 | Hop-interacting protein THI111 | gi|365222920 | Solanum lycopersicum | 121(53) | 44676.5 | 5.1 | 9 | 0.219 |
| Carbohydrate metabolic process | ||||||||
| 14 | alpha-mannosidase | gi|301176645 | Solanum lycopersicum | 179(53) | 116408.8 | 6.18 | 13 | 6.958 |
| 20 | alpha-galactosidase | gi|10312171 | Solanum lycopersicum | 391(53) | 41841 | 4.95 | 27 | 0.444 |
| 26 | chloroplast sedoheptulose-1,7-bisphosphatase | gi|238563983 | Solanum lycopersicum | 433(53) | 42560.6 | 6.07 | 32 | 0.294 |
| 46 | glyceraldehyde 3-phosphate dehydrogenase | gi|2078298 | Solanum lycopersicum | 298(53) | 31926.2 | 5.93 | 14 | 0.196 |
| Ethylene biosynthesis | ||||||||
| 4 | 1-aminocyclopropane-1-carboxylate oxidase homolog | gi|119640 | Solanum lycopersicum | 72(59) | 41050.5 | 5.61 | 10 | 3.252 |
| 6 | 1-aminocyclopropane-1-carboxylic acid oxidase | gi|308390267 | Solanum lycopersicum | 274(53) | 35790 | 5.13 | 33 | 3.002 |
| 7 | Peptide methionine sulfoxide reductase | gi|1709692 | Solanum lycopersicum | 436(53) | 21904.6 | 6.1 | 17 | 3.397 |
| 13 | 1-aminocyclopropane-1-carboxylate oxidase homolog | gi|119640 | Solanum lycopersicum | 122(65) | 41050.5 | 5.61 | 13 | 3.770 |
| 15 | 1-aminocyclopropane-1-carboxylate oxidase homolog | gi|119640 | Solanum lycopersicum | 365(65) | 41050.5 | 5.61 | 22 | 3.440 |
| Hydrogen peroxide catabolic process | ||||||||
| 12 | Suberization-associated anionic peroxidase 2 | gi|129811 | Solanum lycopersicum | 124(53) | 38523 | 4.57 | 6 | 0.120 |
| 19 | catalase | gi|170398 | Solanum lycopersicum | 331(53) | 56470 | 6.56 | 23 | 0.457 |
| 45 | Catalase isozyme 2 | gi|1705616 | Solanum lycopersicum | 79(59) | 56415 | 6.56 | 11 | 3.834 |
| Glycolysis | ||||||||
| 5 | enolase | gi|1161573 | Solanum lycopersicum | 177(53) | 35073.1 | 6.29 | 19 | 2.360 |
| 8 | ethylene-responsive enolase | gi|5669648 | Solanum lycopersicum | 77(53) | 7756.8 | 4.75 | 2 | 2.042 |
| Transport activity | ||||||||
| 9 | ATP synthase CF1 beta chain | gi|89280642 | Solanum lycopersicum | 1120(53) | 53433.9 | 5.28 | 42 | 2.319 |
| 34 | ATP synthase F1 subunit 1 | gi|316996023 | Nicotiana tabacum | 144(59) | 55289 | 5.84 | 12 | 0.070 |
| Calcium ion binding | ||||||||
| 27 | Calmodulin | gi|2388889 | Solanum lycopersicum | 80(65) | 13307.3 | 4.1 | 6 | 0.256 |
| Cell death | ||||||||
| 28 | 14-3-3 protein 9 | gi|15637116 | Solanum lycopersicum | 327(53) | 28902.3 | 4.71 | 25 | 2.330 |
| Purine ribonucleoside salvage | ||||||||
| 31 | adenine phosphoribosyltransferase-like | gi|82621166 | Solanum tuberosum | 71(59) | 19821.6 | 5.17 | 5 | 2.926 |
| Terpenoid biosynthetic process | ||||||||
| 37 | ISPH protein | gi|262176919 | Solanum lycopersicum | 90(53) | 51862.2 | 5.38 | 23 | 2.574 |
| Purine catabolic process | ||||||||
| 38 | chloroplast elongation factor TuB (EF-TuB) | gi|218312 | Nicotiana sylvestris | 105(59) | 46672 | 5.7 | 21 | 3.416 |
| virus-host interaction | ||||||||
| 21 | viral envelope protein | gi|1644306 | Nicotiana tabacum | 76(59) | 6754.4 | 4.72 | 4 | 3.020 |
| Others | ||||||||
| 3 | plastid transketolase | gi|194396261 | Nicotiana tabacum | 103(59) | 80051.4 | 6.16 | 11 | 0.197 |
| 10 | hypothetical protein | gi|2213425 | Citrus x paradisi | 95(65) | 32622.6 | 5.46 | 6 | 2.378 |
| 16 | formate dehydrogenase | gi|56562181 | Solanum lycopersicum | 272(53) | 42066.6 | 6.87 | 34 | 0.375 |
| 24 | Capsid protein | gi|116818 | Solanum lycopersicum | 539(53) | 17734.9 | 4.85 | 7 | 0.070 |
| 25 | BTF3-like transcription factor | gi|83584406 | Solanum lycopersicum | 233(53) | 17397.1 | 6.85 | 11 | 0.325 |
| 29 | At2g16900/F12A24.8 | gi|14532492 | Arabidopsis thaliana | 74(65) | 42969.1 | 5.44 | 10 | 0.058 |
| 32 | alcohol dehydrogenase-2 | gi|170368 | Solanum lycopersicum | 246(53) | 41013.8 | 6.03 | 21 | 0.045 |
| 35 | Capsid protein | gi|10719960 | Solanum lycopersicum | 393(53) | 17750.9 | 4.85 | 11 | 0.017 |
a
Mascot matching score (the threshold value).
b
NUMP is the abbreviation of number of unique matched peptides.
C
Changed expression level in inoculated tissue compared with that in non-inoculated ones.