Skip to main content
. 2013 Aug 15;6(9):1770–1780.

Figure 1.

Figure 1

Generation of GHS-R-/- mice. A: Vector map for gene targeting. PGK-neo on X-pPNT vector was replaced by TK-neo. The 5’ homologous chromosome arm was inserted into BamHI-EcoRI site while 3’ homologous chromosome arm was inserted into NotI-SalI site. B: The schematic diagram of GHS-R gene deletion. The exon 1, exon 2 and a part of exon 3 of GHS-R gene were replaced by TK-neo cassette on X-pPNT vector via homologous recombination. C: PCR identification of the 3’ homologous chromosome arm in recombinant ES cells after GHS-R gene knockout. The 6.4 kbp of PCR products were observed in lane 1, 2, 3 and 4 while another expected 4.2 kbp was observed only in lane 4 indicating the fourth sample (clone No. 120) was the positive clone. D: PCR identification of the 5’ homologous chromosome arm in recombinant ES cells after GHS-R gene knockout. The expected 3.8 kbp of PCR products were observed in all lanes indicating those samples were the positive clones.