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. Author manuscript; available in PMC: 2014 Sep 5.
Published in final edited form as: Eur J Pharmacol. 2013 May 21;715(0):256–261. doi: 10.1016/j.ejphar.2013.05.013

Fig. 3.

Fig. 3

Immunoprecipitated hepatic whole cell lysate and microsomal ubiquitinated CYP2C12 protein from diluent or octreotide female rats. Rats fitted with our chronic indwelling atrial catheter (Pampori et al., 1991) were injected with either octreotide (25μg octreotide/kg body weight) or an equivalent volume of physiologic saline every 12 h for a total of 12 infusions. Hepatic whole cell lysate and microsomes were immunoprecipitated with antibodies against CYP2C12 and subsequently probed with mouse anti-ubiquitin IgG. Relative levels were calculated by comparing all values to the control liver with the highest concentration of ubiquitinated CYP2C12 (i.e., 100%). A representative immunoblot of CYP2C12 ubiquitin is presented in the figure. Values are means ± S.D. with a N=7. *P<0.01 compared to diluent-treated controls of the same cell fraction.