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. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: J Allergy Clin Immunol. 2013 May 17;132(3):593–600.e12. doi: 10.1016/j.jaci.2013.04.005

Figure 2.

Figure 2

TSLP protein was cleaved by NP extracts. Recombinant TSLP was incubated with 1 mg/ml NP tissue extracts or 1 mg/ml BSA (control) for 24–48 hours, and the stability of TSLP was determined by ELISA (A) (n=5). TSLP was incubated with 1 mg/ml BSA or 1 mg/ml NP tissue extracts from donor 1–7 (NP1–7) for 24 hours, and cleavage of TSLP was determined by western blot (B). TSLP was incubated with 1 mg/ml NP extracts for 0–24 hours (C). TSLP was incubated with NP extracts in the presence of 1% protease inhibitor cocktail (PIC) or 1% DMSO (vehicle control) for 24 hours. The results are representative of three separate experiments with separate donors (B–D). * p < 0.05, paired Student t test.