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. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: J Allergy Clin Immunol. 2013 May 17;132(3):593–600.e12. doi: 10.1016/j.jaci.2013.04.005

Figure 3.

Figure 3

NP extract-treated TSLP had higher activity in mast cells. TSLP was preincubated with NP extract for 24 hours. Mast cells were then stimulated with NP-treated TSLP and untreated TSLP in the presence of 20 ng/ml IL-1β for 48 hours (n=8) (A). Mast cells were stimulated with 4 μg/ml NP extracts, 20 ng/ml IL-1β 10 ng/ml TSLP or their combination, but without preincubation, for 48 hours (n=5) (B). Concentration of IL-5 was measured by CBA. *; p<0.05 compared to IL-1β alone (A, red bar) or medium control (B). NS, not significant.