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. 1970 Jan;5(1):1–7. doi: 10.1128/jvi.5.1.1-7.1970

Characterization of Bluetongue Virus Ribonucleic Acid

D W Verwoerd 1, H Louw 1, R A Oellermann 1
PMCID: PMC375961  PMID: 4315158

Abstract

An improved purification procedure yielded bluetongue virus free from any single-stranded ribonucleic acid (RNA) component. Double-stranded RNA obtained from purified virus or isolated from infected cells was fractionated into 5 components by means of sucrose gradient sedimentation analysis, and into 10 components by electrophoresis on polyacrylamide gels. The size of these components vary from 0.5 × 106 to 2.8 × 106 daltons, with a total molecular weight estimate of about 1.5 × 107 for the viral nucleic acid. The denaturation of the genome and separation of the resulting fragments are also discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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