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. 2013 Jun 26;4(7):1008–1018. doi: 10.18632/oncotarget.1049

Figure 3. MMSET regulated the transcription level of SLAMF7 gene.

Figure 3

(A) Cells were treated with shRNAs and cultured for 48 h. QPCR analysis showed SLAMF7 transcription was reduced greatly upon MMSET knockdown. Columns, mean; bars, SD. Average CT values were first normalized against the housekeeping gene b-Actin and converted to the induced fold change relative to the vehicle control. (B) MMSET binding was concentrated upstream of the SLAMF7 promoter (near −1,500 bp). Quantitative ChIP assay indicated that MMSET occupied the 5' end promoter region of SLAMF7 gene in KMS11 cells, while the MMSET occupancy was absent in KMS11/MMSET knockdown cells. MMSET Ab, rabbit IgG against MMSET protein C-terminus. (C) Luciferase reporter assay on SLAMF7 promoter in KMS11 cells. The left panel shows a schematic drawing of various fragments of the SLAMF7 promoter in the luciferase reporter vectors. The promoter length is indicated by the numbering relative to +1 position of transcription start site. Reporter constructs were transfected into KMS11 cells and assayed for luciferase activity. Luciferase activity was normalized to a co-transfected Renilla luciferase reporter. Data represent the mean ± SD derived from 3 separate experiments. * indicates p<0.05, ** indicate p<0.01. ShLuc, control shRNA; Sh#4 and Sh#5, MMSET shRNAs.