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. 2013 Jul 7;4(7):1075–1092. doi: 10.18632/oncotarget.1103

Figure 3. Endogenous TERE1 expression is reduced and cholesterol is elevated in the LnCaP C81 cell model of CRPC.

Figure 3

The LnCaP prostate cancer cell line C81 serves as a model for the Castrate Resistant Prostate Cancer phenotype based on its steroidogenic potential. C81 was derived from the parental C33 clone which is not steroidogenic. Immunoblot analysis with goat anti-TERE1 antibody showed that C81 expresses a reduced level of TERE1 and has a 17% elevated cholesterol level. In lipid free growth media, C33 cholesterol levels are significantly reduced but C81 level remains unchanged, suggesting C33 is more dependent on transport and C81 more dependent on synthesis and/or retention. The cholesterol level of C81 was reduced by lovastatin (10μM) to a greater extent than C33 suggesting that C81 cells have a greater potential for cholesterol synthesis.