Figure 1.

Immune responses of PBMCs from RA patients to cFBG peptides. PBMCs from RA patients (n=17) were stimulated with either non-citrullinated or citrullinated fibrinogen (cFBG) peptides at indicated concentrations (µg/ml). IFN-γ secretion was quantified from culture supernatant of peptide-stimulated PBMCs (A). Similarly, IL-17 secretion was quantified from the supernatant (B). Cell proliferation was evaluated with a dilution of CFSE (C). Representative FACS results are shown (D) (un-stimulation in black [% division: 0.31] and recall antigen stimulation in gray line [% division: 16.5]). ^#p<0.01 compared with non-stimulation (-). ^*p<0.05 compared with non-stimulation. (+) represents a mixture of recall antigens.