Figure 1.

Hypoxia does not activate caspase-1 in BV-2 cells. Cultural supernatants or lysates were immunoblotted as indicated. (A) BV-2 cells were resuspended in glucose-free media and incubated in hypoxic chamber for the indicated times. (B) BV-2 cells were treated with OGD as described in (A) for 5 h in the presence or absence of zVAD (20µM). (C) BV-2 cells were treated with LPS (0.25µg/ml) for 3 h, followed by treatment with CoCl₂ (100µM) in the presence or absence of zVAD (20 µM) for 15 h.