Figure 5.
CoCl2-induced hypoxia reduces NLRP3-dependent caspase-1 activation in primary microglial cells. (A) Flow cytometric analysis of mouse brain mixed glial cells (upper) and isolated microglial cells (lower) using anti-CD11b-PE and anti-F4/80 antigen-APC antibody. (B) Immunoblots for the indicated antibodies in cultural supernatants of brain mixed glial cells or isolated microglial cells treated with LPS (0.25µg/ml) with or without CoCl2 (100µM) for 7 h, followed by treatment with ATP (5 mM, 45 min).