Figure 3.

Cytokine production from monocytes stimulated by soluble PrP^C-Fc treatment. Primary monocytes were cultured with soluble PrP^C-Fc, heat-denatured PrP^C-Fc, or control Fc at the indicated concentrations for 48 h. The culture supernatant was collected, and the concentrations of TNF-α, IL-1β, and IL-6 were determined with the Quantikine Assay Kit. The assay was performed in triplicate. Bar graphs represent the mean±SEM. Statistical analysis was performed in comparison with untreated control. ^*p<0.05; ^**p<0.01. The data are representative of four independent experiments.