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. 2013 Aug 6;2(8):e113. doi: 10.1038/mtna.2013.38

Figure 3.

Figure 3

Detection of viral genomic DNA and LATs. (a) MGH2.1 (5 × 107 pfus) ± CPA (intraperitoneally (i.p.) at 1, 3, 5, and 7 days) or CPT11 (i.p. at 1 day) was intracerebrally injected in mice and viral genomic DNA was isolated from brain and TG 7 days after virus inoculation. (b) MGH2.1 (107 and 108 pfus) was intracerebrally injected in mice and viral genomic DNA was isolated from brain and TG 60 days after virus inoculation. PCR for viral DNA pol was carried out by 35-cycle amplification, and amplified products were separated by agarose gel electrophoresis. Mouse β-actin was used as an internal control for genomic DNA. (c) Expression of transgenes (rat CYP2B1 and human shiCE) and LAT were analyzed by reverse transcription-PCR 60 days after intracerebral inoculation of MGH2.1 or PBS. Mouse GAPDH was used as an internal control for mRNA. CPA, cyclophosphamide; CYP2B1, cytochrome P4502B1; LAT, latency-associated transcript; PBS, phosphate-buffered saline; pol, polymerase; shiCE, secreted human intestinal carboxylesterase; TG, trigeminal ganglia.