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. 2013 Aug 13;2(8):e115. doi: 10.1038/mtna.2013.40

Figure 2.

Figure 2

Restoration of TGF-β signaling in UOK257-FS cells. The compilation of protein bands from separate Western blots show the (a) upregulation of SMAD3 and pSMAD3 expression in stable UOK257-FS cells (2) compared with stable UOK257-Luc cells (1), untreated UOK257 cells (3) and UOK257 cells following transient 24 hours transfection with pUbC-FLCN-SMAR (4). (b) Upregulation of SMAD3, SMAD7, and TGFβ2 mRNA levels in UOK257-FS cells relative to endogenous UOK257 mRNA levels. No differences in SMAD3, SMAD7, and TGFβ2 levels were detected in UOK257-Luc cells over UOK257 mRNA levels. Columns represent mean ± SEM (n = 3). (c) Significantly higher levels of secreted TGFβ2 protein in measured the medium of UOK257-FS cells compared with UOK257 cells (*P < 0.05), as measured by ELISA.