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. 2013 Sep 3;4:111. doi: 10.3389/fphar.2013.00111

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Copyright © Kaufman, Gordon, Bergamaschi, Wang, Cohen, Valiunas and Brink.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) A pipette containing 2 mmol/L LY was attached to the cell furthest to the left in whole cell configuration. Epi-fluorescent micrographs were taken at 1, 5, and 10 min after dye injection into the first cell and showed a progressive fluorescence intensity increase in the two adjacent recipient cells. Scale bar: 20 μm. (B) Quantification of cell-to-cell spread of LY. Fluorescence intensity plots versus time for the 5 mM 4-PB HeLa Cx43 cell trial shown in (A): first (injected) cell (▲), second cell (■), and third cell (•).