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. 2013 Sep 3;4:111. doi: 10.3389/fphar.2013.00111

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Copyright © Kaufman, Gordon, Bergamaschi, Wang, Cohen, Valiunas and Brink.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Western blot analysis of HEK-293 (left panel) and HeLa Cx43 (right panel) cells using polyclonal Cx43 antibody. Lines from left to right correspond to 0, 1, 2, and 5 mM 4-PB, respectively. Anti-α tubulin is shown as a reference indicator. (B) Summary of Western blot data from four experiments with HEK-293 control cells and cells exposed to 5 mM 4-PB. Upon exposure to 5 mM 4-PB Cx43 expression increased to 166 ± 11% which was statistically significant (p = 0.029) in comparison to the control cells (0 mM 4-PB) where Cx43 expression was assumed to be 100%.