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. 2013 Sep 3;7:135. doi: 10.3389/fncir.2013.00135

Figure 6.

Figure 6

THP exerts polarity-dependent effects at α4βδ GABAA receptors. THP, a neurosteroid, decreases hyperpolarizing current gated by α4βδ GABAA receptors expressed in HEK-293 cells (recorded with whole cell voltage clamp techniques). (A) THP effects on hyperpolarizing and depolarizing currents in response to 1 μ M GABA (upper panel) or the GABA EC20 (lower panel; α4β2δ, 0.1 μ M; α4β2γ2, 5 μ M; α1β2γ2, 10 μ M; α5β3γ2, 5 μ M) from 6–7 cells for each group (Mean ± SEM; *P = 0.05 vs. the other receptor subtypes). n = 6–8 cells/group. (B) Effects of THP (30 nM) on current in response to a voltage ramp over 400 ms where the ECl = 0 mV. THP increases depolarizing current (voltage < 0 mV) but decreases hyperpolarizing current (voltage > 0 mV). (Leak-subtracted current is presented as the average of three traces). n = 5–6 cells/group. (C) Effects of THP on desensitization of hyperpolarizing (upper traces) and depolarizing (lower traces) current at α4βδ receptors (representative of 6 cells per group). THP accelerates desensitization of the hyperpolarizing current. [Used with permission (Shen et al., 2007)].