Figure 3.

Expression of IL-1β mRNA and pro IL-1β in macrophages is not influenced by the genotype of M. tuberculosis. Macrophages were infected with M. tuberculosis at a MOI of 5 and (A) expression of IL-1β mRNA at 6 h and 24 h post-infection was determined using real-time PCR. Fold change represents increased IL-1β transcript expression over uninfected cells (B) Levels of pro IL-1β was assayed by western blot at 6 h and 24 h in the lysates of M. tuberculosis infected macrophages. For the real-time PCR experiments, data represents means ± SD of two independent experiments.