Figure 5.

IL-1β secretion is partially dependent on caspase-1. Macrophages were infected with M. tuberculosis at a MOI of 5 and (A) levels of pro-caspase-1 were measured by western blot at 6 h and 24 h. Infected macrophages were treated with 20 μM caspase-1 inhibitor (Ac-YVAD-AOM) or treated with 5 μM Nigericin individually or in succession. Cytokine concentrations for IL-1β was analysed in the supernatants by ELISA at 6 h (B) and 48 h (C). The absolute values of IL-1β are expressed as a fold change over control strains.