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. 2013 Jul 25;14(8):15459–15478. doi: 10.3390/ijms140815459

Figure 3.

Figure 3

BDNF increases cell migration and MMP-1 expression through the TrkB receptor. (A–E) Cells were pretreated with K252a (50 nM) for 30 min followed by stimulation with BDNF (50 ng/mL), and in vitro migration, invasion, and MMP-1 expression levels were measured using Transwell, qPCR, ELISA, and western blotting; (F) The protein levels of TrkB and MMP-1, and the in vitro migration activity of JJ012/control-shRNA and JJ012/TrkB-shRNA cells were measured by western blotting and Transwell assays. Results are expressed as the mean ± S.E. *p < 0.05 compared with control; #p < 0.05 compared with the BDNF-treated group.