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. 2013 Jul 25;14(8):15459–15478. doi: 10.3390/ijms140815459

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© 2013 by the authors; licensee MDPI, Basel, Switzerland

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

PMC Copyright notice

BDNF increases cell motility and MMP-1 expression through the p38 and c-jun N-terminal kinase (JNK) pathways. (A) JJ012 cells were transfected with dominant negative (DN) mutants of p38 and JNK for 24 h, and p38 and JNK phosphorylation were examined by western blotting; (B–F) Cells were pretreated for 30 min with SB203580 (10 μM) and SP600125 (10 μM) or were transfected with DN mutants of p38 and JNK for 24 h followed by stimulation with BDNF (50 ng/mL), and in vitro migration and invasion or MMP-1 expression were measured by Transwell, qPCR, ELISA, and western blotting; (G) JJ012 cells were incubated with BDNF (50 ng/mL) for the indicated time intervals, and p38 and JNK phosphorylation were examined by western blotting; (H) JJ012 cells were transfected with TrkB or ASK1 shRNA for 24 h followed by stimulation with BDNF (50 ng/mL) for 30 min, and p38 and JNK phosphorylation were determined by western blotting. Results are expressed as the mean ± S.E. *p < 0.05 compared with control; ^#p < 0.05 compared with the BDNF-treated group.

BDNF increases cell motility and MMP-1 expression through the p38 and c-jun N-terminal kinase (JNK) pathways. (A) JJ012 cells were transfected with dominant negative (DN) mutants of p38 and JNK for 24 h, and p38 and JNK phosphorylation were examined by western blotting; (B–F) Cells were pretreated for 30 min with SB203580 (10 μM) and SP600125 (10 μM) or were transfected with DN mutants of p38 and JNK for 24 h followed by stimulation with BDNF (50 ng/mL), and in vitro migration and invasion or MMP-1 expression were measured by Transwell, qPCR, ELISA, and western blotting; (G) JJ012 cells were incubated with BDNF (50 ng/mL) for the indicated time intervals, and p38 and JNK phosphorylation were examined by western blotting; (H) JJ012 cells were transfected with TrkB or ASK1 shRNA for 24 h followed by stimulation with BDNF (50 ng/mL) for 30 min, and p38 and JNK phosphorylation were determined by western blotting. Results are expressed as the mean ± S.E. *p < 0.05 compared with control; ^#p < 0.05 compared with the BDNF-treated group.