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Dominant PCR markers for triticale HMW glutenin subunits (a) Dx5/Dx2; (b) AxNull; and (c) By20*. Primer set GS I produces a 450 bp fragment in triticale lines that have the Glu-D1d (Dx5) allele. The PCR product for the Glu-A1c (AxNull) allele is 920 bp and that for the By20* subunit is 750 bp. Lanes, M, 100-bp DNA Ladder Plus (Promega); 1, Glu-Sec group; 2, Glu-Sec + Tm; 3, Glu-Sec + 1D; 4, Glu-Sec + Tm + 1D group.
