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. 2013 Jul 26;14(8):15595–15614. doi: 10.3390/ijms140815595

Figure 4.

Figure 4

CZE electrophorograms of nonalkylated HMW proteins from four distinct introgressive Triticale/Triticum monococcum groups with various HMW-GS and HMW-SS compositions and reference triticale and wheat cultivars. (a) standard triticale (cv. Lasko; 2*/6.8 + 20*/2r + 5.3r); (b) Glu + Sec (N/6.8 + 20*/2r + 5.3r); (c) Glu + Sec + Tm (N/6.8 + 20*/2r + 5.3r; 5.2 m + 14 m); (d) Glu + Sec + 1D (N/6.8 + 20*/2r + 5.3r; 5 + 10); (e) Glu + Sec + Tm + 1D (N/6.8 + 20*/2r + 5.3r; 5.2 m + 14 m; 5 + 10); (f) Standard wheat (cv. Panda) (1/7 + 9/5 + 10). The HMW-SS and HMW-Tm peaks are additionally indicated by the letter “r” and “m”, respectively, at the end of sing. The proteins were separated at 10 kV and 40 °C with a 75 mM iminodiacetic acid (IDA), 0.15% poly(ethylene oxide) (PEO) with a molecular weight of 8,000,000, 0.05% 26 mM SB3-12, and 20% (v/v) acetonitrile.