Table 4.
Sequences of primers used.
| Genes | Forward primer | Reverse primer |
|---|---|---|
| Oxidative stress | - | - |
| SOD-3 | CTTCCCAGCCGAGCAGAA | TCAGGTCCCCGAACTCATG |
| CAT | TCCTGAGAGAGTGGTACATGC | AAGGTGTGAGCCATAGCC |
| GPX-1 | AATTCCCTCAAGTACGTCCG | TCAGGTTCGATGTCGATGGT |
| NOX-4 | TGGGTGTCAAACAAGAGATTGC | GGCTCAGGAGGTTCTTCATGTAG |
| DNA Damage | - | - |
| p300 | GGGACTAACCAATGGTGGTG | ATTGGGAGAAGTCAAGCCTG |
| APEX-1 | GAATGTGGATGGGCTTCGA | AAGATGTCTGGTGCTTCTTCCTTT |
| OGG-1 | CGGCTGGCAGCCTAAGAC | CCGGAAAAAGTTTCCCAGTTC |
| p53 | GACAGCTTTGAGGTTCGTGTTTG | TGCTCTTCTTTTTTGCGGAAA |
| Inflammation | - | - |
| PAI-1 | TCCGCCATCACCAACATTTT | GTCAGTCATGCCCAGCTTCTC |
| TNFα | TGATCGGTCCCAACAAGGA | GGGCCATGGAACTGATGAGA |
| IL-1β | GCACCTTCTTTTCCTTCATCTTTG | CACACTAGCAGGTCGTCATCATC |
| IL-6 | GAAACCCTAGTTCATATCTTCAAACAAG | CCATTAGGAGAGCATTGGAAGTTG |
| Fibrosis | - | - |
| CTGF | CACAGAGTGGAGCGCCTGTTC | GATGCACTTTTTGCCCTTCTTAATG |
| Col1α1 | CGAAGGCAACAGTCGATTCA | GGTCTTGGTGGTTTTGTATTCGAT |
| MMP-2 | TTTGCTCGGGCCTTAAAAGTAT | CCATCAAACGGGTATCCATCTC |
| MMP-9 | GACCTGAAAACCTCCAACCTC | CTGCTTCTCTCCCATCATCTG |
| Hypertrophy | - | - |
| ANP | ATCACCAAGGGCTTCTTCCT | TGTTGGACACCGCACTGTAT |
| BNP | AGACAGCTCTCAAAGGACCA | CTATCTTCTGCCCAAAGCAG |
| Telomere length | - | - |
| PGK1 | CGGAGACACCFCCACTTG | AAGGCAGGAAAATACTAAACAT |
| Telomere 1 | CGGTTTGTTTGGGTTTGGGTTTGGGTTTGGGTTTGGGTT | |
| Telomere 2 | GGCTTGCCTTACCCTTACCCTTACCCTTACCCTTACCCT | |
| Reference gene | - | - |
| β-actin | GACAGGATGCAGAAGGAGATTACTG | CCACCGATCCACACAGAGTACTT |