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. 2013 Aug 6;14(8):16226–16239. doi: 10.3390/ijms140816226

Figure 5.

Figure 5

Early growth response 2 (EGR2) was a direct target of miR-20a. (A) Wild type of the mutated sequences of EGR2 3′ UTR (nucleotides 458-465); (B) SGC7901 cells were co-transfected with miR-20a precursor or negative control (miR-NC) with EGR2 3′ UTR fragment with either the miR-20a target sequence (WT), or a mutant (Mut). Luciferase activity was detected; (C) EGR2 protein level was detected by Western blot in cells transfected with miR-20a precursor/inhibitor or the corresponding control; (D) expression of EGR2 mRNA was detected by qRT-PCR in cells transfected with miR-20a precursor/inhibitor or the corresponding control. GAPDH was used as an internal control. The data represented at least four measurements. *p < 0.05 compared with control.