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Thrombin activity is increased after OGD. Thrombin enzyme activity in hippocampal slice culture homogenates was assayed using a chromogenic substrate with purified recombinant mouse thrombin as a standard. The results were expressed as units of thrombin/mg of protein. Thrombin enzyme activity is significantly (Kruskall Wallis test p = 0.0006, followed by Mann-Whitney test p < 0.05) increased in hippocampal slice cultures 24 h after oxygen and glucose deprivation (OGD). (* p < 0.05).
