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. 2013 Aug 14;14(8):16719–16731. doi: 10.3390/ijms140816719

Figure 3.

Figure 3

TPC enhances PN-1 transcription in vitro. Organotypic hippocampal slice cultures from PN-1 KI mice expressing the β-galactosidase protein under the control of the PN-1 promoter were used. X-gal staining reflecting β-galactosidase activity was detected and quantitated in the stratum radiatum. The stratum radiatum was identified by double labellling with X-gal and the neuronal marker Neu N, which labels the neuronal layers. The major site of X-Gal labelling did not coincide with Neu N labelling suggesting glial cell labelling. Compared to controls (A), OGD (B) significantly enhanced PN-1 expression. TPC alone (C) further enhanced the expression of PN-1 (ANOVA followed by Tukey-Cramer, p < 0.001, compared to OGD alone). If OGD was preceded by TPC, (D), the expression was significantly higher than with OGD alone (ANOVA followed by Tukey-Cramer, p < 0.001). N represents the total number of slices. A high magnification image of X-gal stained cells is inserted into the right-hand corner of image B. (** p < 0.01; *** p < 0.001).