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. 2013 Aug 19;14(8):16999–17016. doi: 10.3390/ijms140816999

Figure 5.

Figure 5

Correlation between the results of the luciferase experiments conducted with miR-137-coding plasmid and synthetic miR-137 mimics. (A) Relative repression of the luciferase expression. Reporter constructs carrying a single binding site for miR-137 were tested; miRNA activity on four constructs was measured in parallel (Control, WT, MUT, and PM), as described in Figure 3. Left—miRNA expression from the synthetic oligonucleotide (miR-137 mimic), right—miRNA overexpressed from the miR-137 vector. The firefly luciferase activity was normalized against Renilla luciferase activity. The standard errors are calculated from three independent experiments; (B) The relative repression of the luciferase expression resulted from the miRNA mimic activity. Four reporter constructs were tested (Control, WT, MUT, and PM) but with the addition of miR-137 mimic at different final concentrations, specifically 10, 30, and 50 nM, as denoted in the figure. The standard errors were calculated from one experiment performed in triplicate.