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Caspase-3 activity in SAS cells by CuE 24 h treatment. (a) The cells were treated with CuE (0, 1.25, 2.5 and 5 μM) for 24 h and procaspase-3 proteins were subsequently detected by Western blot analysis; (b) Following treatment, the cells were harvested and labeled using FITC rabbit anti-active caspase-3. Activation was quantified by flow cytometry; and (c) Quantification by flow cytometry. All data was reported as the mean (±SEM) of at least three separate experiments.
