Figure 5. Effect of RES and PTS on ARE-hNQO1 and ARE-GST luciferase activation systems.
HEK293T cells were transfected with either ARE-hNQO1 or ARE-GST luciferase transgene. Transfected cells were treated with RES (10μM) and PTS (10μM) for 8 h. Luciferase assay was performed as described in methods. Luciferase activities were expressed as fold induction relative to values obtained from control cells. Photon flux data was normalized to total protein and Rluc expression per well.