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. 2013 Sep;33(9):529–541. doi: 10.1089/jir.2012.0054

FIG. 4.

FIG. 4.

Overexpression of Mfn-2 attenuated HMGB1-induced immune dysfunction of Jurkat cells. Jurkat cells transfected with Lv-Mfn-2 or Lv-GFP (MOI=50) were incubated with PMA (50 ng/mL) plus ionomycin (1 μM) for 12 h, and then stimulated with HMGB1 (100 ng/mL for 24 h) or without HMGB1. Methyl-thiazolyl-tetrazolium cell proliferation assay was used to assess Jurkat cell proliferative activity after HMGB1 stimulation. In addition, levels of IL-2, IL-4, as well as IFN-γ were determined by enzyme-linked immunosorbent assay. Mean values of four individual experiments were shown as mean±standard deviation. *Statistically significant difference when compared with control group (P<0.05). #Statistically significant difference when compared with HMGB1-treated group (P<0.05).