FIG. 4.
Gene expression of the adipogenic differentiated BMAPs. (A) Adipogenic genes' expression on 4 days' differentiated BAMPs (P8). Adipogenic genes involved PPAR-γ, FABP4, C/EBPα, adiponectin, Pref-1, and Glut-4; GADPH was used as the positive control. (B) Real-time PCR analysis of adipogenic genes in P8 BMAPs (black) and NS-BMSCs (white) before and during 4 days' adipogenic differentiation (day0–day4). Gene expression levels were normalized to the expression of GADPH. Box in (B) indicated the significant difference of basal FABP4 gene expression levels. **P<0.01. Pparg, peroxisome proliferator-activated γ; Fabp4, fatty acid-binding protein 4; Cebpa, CCAAT enhancer-binding protein alpha; Adipog, adiponetin; Pref1, preadipocyte factor 1; Glut4, glucose transporter 4; Gapdh, glyceraldehyde 3-phosphate dehydrogenase.