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Paxilline biosynthesis gene replacements. (a) Physical maps of P. paxilli wild-type genomic region, linear replacement construct and mutant allele for each of paxA (PP114), paxM (PP115), paxB (PP116), paxC (PP117) and paxD (PP120); (b) Autoradiographs of Southern blots of 1 μg genomic digest of P. paxilli wild-type and mutant alleles, probed with [32P] dCTP-labeled replacement construct for each gene.
