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. 2013 Aug 14;5(8):1422–1446. doi: 10.3390/toxins5081422

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© 2013 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

PMC Copyright notice

Coordinate expression of the pax genes is correlated with the onset of paxilline biosynthesis. (a) Time course of paxilline biosynthesis. For paxilline extraction, wild-type mycelium was harvested between 24 and 132 h of inoculation at 12 h intervals; (b) Expression analysis of pax genes. Total RNA was isolated from wild-type mycelium for each time point and used for cDNA synthesis. RT-PCR was performed with primers specific for each of the pax genes as well as the ORFs PP111, PP112, PP121, PP122 and tub2 (β-tubulin).