FIG. 2.

Ddx46 expression in HSCs. The expression of Ddx46 and cmyb in wild-type larvae was examined by whole-mount in situ hybridization at 2, 3, and 4 dpf. All are lateral views, anterior to the left. (A-C) Ddx46 is ubiquitously expressed in the AGM (arrowheads in A) and CHT (arrow in A) at 2 dpf (n=6/6) and is specifically expressed in the CHT (boxed area in B) at 3 dpf (n=6/6). The boxed area in (B) is shown enlarged in (C). (D, E) The transcripts of both genes (Ddx46, n=11/11; cmyb, n=9/9) were detected in the CHT at 4 dpf. (F) Double whole-mount in situ staining showed that the expression domains of Ddx46 (blue) and cmyb (red) overlapped in the CHT (arrows) (n=7/7) at 4 dpf. Scale bars represent 50 μm. HSCs, hematopoietic stem cells; AGM, aorta-gonad-mesonephros; CHT, caudal hematopoietic tissue. Color images available online at www.liebertpub.com/scd