FIG. 3.
Expression of cmyb and tal1 in Ddx46hi2137/hi2137 mutants is rescued by overexpression of Ddx46 mRNA. (A–F) The expression of cmyb and tal1 was examined by whole-mount in situ hybridization at 3 dpf. All are lateral views, anterior to the left. The number of cmyb- or tal1-expressing HSCs in the EGFP mRNA-injected Ddx46hi2137/hi2137 larvae (cmyb, 0 of 26 EGFP mRNA-injected mutants were rescued; tal1, 0 of 21 EGFP mRNA-injected mutants were rescued) was markedly lower than that in the EGFP mRNA-injected Ddx46hi2137/+ larvae (cmyb, n=17/17; tal1, n=16/16) at 3 dpf (A, B, D, E). The overexpression of Ddx46 mRNA was able to rescue the number of cmyb- or tal1-expressing HSCs in Ddx46hi2137/hi2137 larvae (cmyb, 19 of 21 Ddx46 mRNA-injected mutants were rescued; tal1, 19 of 22 Ddx46 mRNA-injected mutants were rescued) at 3 dpf (B, C, E, F). Scale bars represent 100 μm. Color images available online at www.liebertpub.com/scd