FIG. 4.

Number of cmyb-expressing cells decreases in Ddx46^{hi2137/hi2137} mutants. (A–G) The expression of cmyb was examined by whole-mount in situ hybridization at 36 hpf, 48 hpf, or 3 dpf. All are lateral views, anterior to the left. The number of cmyb-expressing HSCs in the AGM (arrowheads) and CHT (arrows) was counted at 36 hpf, 48 hpf, and 3 dpf (G). At 36 hpf, the number of cmyb-expressing HSCs in the AGM (arrowheads) was indistinguishable between Ddx46^hi2137/+ and Ddx46^{hi2137/hi2137} larvae (A, B, G). In contrast, the number of cmyb-expressing HSCs in the CHT (arrows), but not in the AGM (arrowheads), at 48 hpf (C, D, G) and in both the AGM (arrowheads) and CHT (arrows) at 3 dpf (E, F, G) of Ddx46^{hi2137/hi2137} larvae was significantly reduced compared with that of Ddx46^hi2137/+ larvae. Ddx46^hi2137/+ larvae: n=13/13 (36 hpf), n=18/18 (48 hpf), n=13/13 (3 dpf); Ddx46^{hi2137/hi2137} larvae: n=9/9 (36 hpf), n=14/14 (48 hpf), n=11/11 (3 dpf). Black dotted lines in (C–F) indicate the boundary between the AGM and CHT. Error bars represent the standard error. *P<0.01 by the Student's t-test. Scale bars represent 100 μm. Color images available online at www.liebertpub.com/scd