FIG. 5.

Cell death is not upregulated in the CHT of Ddx46^{hi2137/hi2137} mutants. (A–F) Confocal microscopic images of dead cells (red) detected by the TUNEL method at 48 hpf, 3 dpf, or 4 dpf. All are lateral views, anterior to the left. The white, boxed regions show an area of the CHT. (G) The number of labeled cells in the white, boxed regions (A–F) was counted. The number of dead cells in the CHT was indistinguishable between Ddx46^hi2137/+ and Ddx46^{hi2137/hi2137} larvae at 48 hpf, 3 dpf, and 4 dpf. Ddx46^hi2137/+ larvae: n=7/7 (48 hpf), n=5/5 (3 dpf), n=5/5 (4 dpf); Ddx46^{hi2137/hi2137} larvae: n=5/5 (48 hpf), n=6/6 (3 dpf), n=8/8 (4 dpf). Error bars represent the standard error. Scale bars represent 75 μm. Color images available online at www.liebertpub.com/scd