FIG. 6.

Cell proliferation is not downregulated in the CHT of Ddx46^{hi2137/hi2137} mutants at 48 hpf. (A–D) Confocal microscopic images of EGFP fluorescence (green) and anti-PCNA (red) whole-mount immunostaining of the CHT in Ddx46^hi2137/+;Tg(tal1:EGFP) and Ddx46^{hi2137/hi2137};Tg(tal1:EGFP) larvae at 48 hpf. All are lateral views, anterior to the left. Merged single-slice images of cells expressing EGFP (HSCs) and PCNA (proliferating cells). The boxed areas in (A) and (B) are shown enlarged in (C) and (D), respectively. (E) Quantification of the experiments in panels (C) and (D) was performed by plotting the ratio of EGFP⁺ and PCNA⁺ cells (yellow) to the total number of EGFP⁺ cells (green and yellow). No significant difference between Ddx46^hi2137/+;Tg(tal1:EGFP) and Ddx46^{hi2137/hi2137};Tg(tal1:EGFP) larvae was observed. Cells were counted from four single slices from four embryos for each condition. Error bars represent the standard error. The scale bar represents 20 μm. PCNA, proliferating cell nuclear antigen. Color images available online at www.liebertpub.com/scd