FIG. 7.

Chelatable Zn present in Neo-Timm staining. The Neo-Timm staining is indicated by arrows. Neo-Timm and cresyl violet counterstaining of the central nucleus of the dorsal telencephalic area (Dc) (A) and valvula cerebelli, lateral part (Val) (B) of controls. Note that pretreatment with 5 mM TPEN decreased the Neo-Timm staining on the cell bodies in the Dc (C) and slightly reduced positive granules in neuropil regions, for example, in the Val (D). Administration i.p. of DEDTC 700 mg/kg did completely abolish the silver staining on the cell bodies (E) and in neuropil regions (F). The use of 0.1 M HCl in the preparation of the slices did significantly reduce Neo-Timm staining on the cell bodies and in neuropil regions, as observed in the Dc (G) and Val (H), respectively. Color images available online at www.liebertpub.com/zeb