Table 1.
Neo-Timm Staining in Zebrafish Central Nervous System Structures
| Staining level | Structures | Abbreviations | Optic densitya |
|---|---|---|---|
| High | Valvula cerebelli, lateral part (granular layer) | Val | 58.60±6.14 |
| Central gray | CG | 53.25±9.26 | |
| Periventricular gray zone of the optic tectum | PGz | 50.80±4.31 | |
| Eminentia granularis | EG | 48.79±3.81 | |
| Anterior thalamic nucleus | A | 48.23±7.54 | |
| Torus longitudinalis | TL | 45.57±6.75 | |
| Ventral zone of the hypothalamic periventricular nucleus | Hv | 44.49±2.91 | |
| Central preglomerular nucleus | PGc | 42.66±6.08 | |
| Ventromedial thalamic nucleus | VM | 42.41±7.13 | |
| Dorsal zone of the hypothalamic periventricular nucleus | Hd | 41.45±4.87 | |
| Valvula cerebelli, medial part (granular layer) | Vam | 41.07±2.60 | |
| Glossopharyngeal lobe | LIX | 40.62±8.81 | |
| Posterior part of the parvocellular preoptic nucleus | PPp | 39.75±3.38 | |
| Dorsal tegmental nucleus | DTN | 39.59±3.20 | |
| Perilemniscal nucleus | PL | 38.38±1.94 | |
| Magnocellular preoptic nucleus | PM | 37.20±6.89 | |
| Lobus caudalis cerebelli | Lca | 37.11±3.86 | |
| Corpus cerebelli | Cce | 35.62±2.50 | |
| Intermediate | Entopeduncular nucleus, ventral part | Env | 33.99±4.68 |
| Habenula | H | 33.22±8.10 | |
| Periventricular nucleus of the posterior tuberculum | PTp | 30.19±1.86 | |
| Secondary octaval population | SO | 29.56±3.81 | |
| Corpus mamillare | CM | 28.23±4.65 | |
| Secondary gustatory nucleus | SGN | 27.65±3.93 | |
| Medial octavolateral nucleus | MON | 26.76±1.75 | |
| Ventrolateral optic tract | Vot | 25.75±1.07 | |
| Internal cellular layer | ICL | 25.53±4.81 | |
| Oculomotor nucleus | NIII | 24.37±2.85 | |
| Suprachiasmatic nucleus | SC | 23.38±5.64 | |
| Ventral part of the lateral funiculus | Flv | 22.56±6.02 | |
| Nucleus taeniae | NT | 22.09±6.57 | |
| Posterior nucleus of the dorsal Telencephalic area | Dp | 21.89±2.67 | |
| Ventrolateral thalamic nucleus | VL | 20.90±2.33 | |
| Ventral part of the entopeduncular nucleus | Nev | 20.61±2.48 | |
| Dorsal nucleus of the dorsal telencephalic area | Dd | 20.24±2.43 | |
| Anterior part of the parvocellular preoptic nucleus | Ppa | 20.18±2.38 | |
| Subcommissural nucleus | SCN | 19.09±3.87 | |
| Facial lobe | LVII | 18.85±1.45 | |
| Nucleus of the lateral lemniscus | NLL | 18.08±2.54 | |
| Ventral funiculus | Fv | 17.56±2.46 | |
| External cellular layer | ECL | 17.55±2.18 | |
| Low | Ventrolateral nucleus of the torus semicircularis | TSvl | 16.74±2.72 |
| Diffuse nucleus of the hypothalamic inferior lobe | DIL | 15.78±2.02 | |
| Central nucleus of the hypothalamic inferior lobe | CIL | 15.78±2.02 | |
| Nucleus lateralis valvulae | NLV | 15.33±2.73 | |
| Lateral nucleus of the dorsal telencephalic area | Dl | 15.16±2.11 | |
| Glomerular layer | GL | 14.90±5.32 | |
| Optic tectum | OT | 14.94±1.56 | |
| Mesencephalic nucleus of the trigeminal nerve | MNV | 14.22±3.75 | |
| Ventral horn | VH | 14.21±1.13 | |
| Ventral part of the periventricular pretectal nucleus | PPv | 14.02±5.42 | |
| Central nucleus of the torus semicircularis | TSc | 12.36±2.21 | |
| Dorsal part of the lateral funiculus | Fld | 11.38±4.06 | |
| Crista cerebellaris | CC | 11.36±1.83 | |
| Medial nucleus of the dorsal telencephalic area | Dm | 11.06±2.14 | |
| Dorsal part of the entopeduncular nucleus | Ned | 10.50±1.79 | |
| Supracommissural nucleus of the telencephalic ventral area | Vs | 10.18±1.48 | |
| Torus lateralis | Tla | 7.05±1.11 | |
| Central nucleus of the dorsal telencephalic area | Dc | 2.75±0.51 |
Values are presented as mean±SEM in arbitrary units (n=3–6).
a
Mean optical density of the correspondent CNS regions (e.g., PGz1, PGz2…PGz5) in the same stained brain, considering three to six independent experiments.