FIG. 4.
Reperfusion-induced Ψmito hyperpolarization depends on IK,ATP open probability. (A) Changes in TMRM fluorescence during the simulated I/R protocol normalized to the fluorescence during preischemia is shown for VMs reperfused with Ctrl solution supplemented with 200 μmol/L diazoxide (DZX) (−: n=7) or ConT supplemented with 500 μmol/L 5-hydroxydecanoate (5-HD) (black circles: n=6). (B) Bar graph presents normalized TMRM fluorescence at 5 min of reperfusion (R5′). (C) Change in TMRM fluorescence during superfusion with 5-HD (top) or DZX (bottom). (D) Normalized TMRM fluorescence during the I/R protocol. VMs were reperfused with Ctrl solution (gray circles: n=5) or ConT (black circles: n=5) supplemented with the permeability transition pore (PTP) inhibitor cyclosporin A (1 μmol/L). Data are summarized in (E) [*P<0.05 compared with pI; &P<0.05 compared with Ctrl+DZX at 5 min of reperfusion (R5′)].