FIG. 5.
Scavenging of intra-mitochondrial ROS enhances cell survival on I/R. (A) Changes in TMRM fluorescence during the simulated I/R protocol normalized to preischemia for VMs reperfused with Ctrl+mitoTempo (circles: mitoT: 5 μmol/L; n=6; &P<0.05 compared with Ctrl at 5 min of reperfusion). Mean fluorescence of Ctrl-treated cells is shown as reference (−). MitoTEMPO prevents the reperfusion-induced increase in TMRM fluorescence (B). Time-dependent cell survival on reperfusion with Ctrl, ConT, or Ctrl+mitoTempo presented as a Kaplan–Meier curve (C). Bar graph reflecting the number of EADs at 5 and 10 min of reperfusion in the conditions indicated (D). (*,&,#P<0.05 compared with Ctrl, ConT, and mitoT at R1′, respectively).