FIG. 2.

ABA binding to the SEL7 aptamer population (aptamers obtained after the seventh round of selection) compared with the starting ssDNA library. Bio-ABA or the bio-linker were plated at 1.6 nmol/well in 96-well plates and incubated with 8 nM of digoxigenin-labeled aptamers from the SEL 7 population (white bars) or the starting library (RND40). After washing of excess, unbound aptamers, anti-digoxigenin antibodies coupled to horseradish peroxidase (HRP) were added and the reaction was revealed with the 2,2′-azinobis [3-ethylbenzothiazoline-6-sulfonic acid]-diammonium salt (ABTS) solution. Results are the mean of three different experiments, performed in triplicate. *p<0.5, by t-test.