FIG. 6.

Affinity elution assays on the cloned aptamers. The indicated cloned, digoxigenin-labeled aptamers were first incubated with bio-ABA immobilized on magnetic beads (0.6 nmol/well), then challenged without (control) or with an excess (60 nmol/well) of the indicated target molecules in solution. The amount of aptamer retained on the beads was determined by immunoenzymatic reaction (see Methods). Results are expressed as the ratio between the absorbance at 405 nm in the presence and in the absence of the target molecules. [bio-ABA: biotinylated abscisic acid; C/TABA±: (±)2-cis,4-trans-ABA; C/TABA+: (+)2-cis,4-trans-ABA; C/TABA-: (–)2-cis,4-trans-ABA; analog 10: 2′α, 3′α-dihydro-2′α, 3′α–epoxy abscisic acid; bio-linker: (+)-biotin-ɛ-aminocaproyl hydrazide; ±T/TABA: (±)2-trans,4-trans ABA]. *p<0.05 compared with bio-ABA; **p<0.005 compared with bio-ABA, by t-test.